Finding Specimens for the Foldscope

Notes of Caution

While collecting specimens for this lab is a low-risk activity, you are responsible for using common sense and due caution. You should be able to collect and prepare interesting specimens without entering bodies of water or using

potentially dangerous tools such as blades or scissors. Collect only in a location and manner that you judge to be safe, and do not risk injury or misadventure for the sake of this lab.

Please DO:

• wash your hands as soon as possible after your collecting trip
• wash any extra non-disposable equipment that you may have used
• dispose of any disposable equipment you may have used in the appropriate manner (compost, recycling, landfill, etc.)
• pick up some garbage while you're out exploring and dispose of it properly (that's mainly what the gloves are for...)
• tell someone where you're going - or, better yet - bring a friend!

 Please DO NOT:

• enter the ocean or lakes, or anywhere where there is moving water (flowing streams, etc.)
• trespass on private property
• collect specimens where you feel unsafe or where it is unsafe to walk
• walk on submerged rocks, or on rocks covered in algae (whether submerged or not)
• use blades or other sharp objects to prepare your specimens
• handle wild mushrooms (see 'Other Specimens to Try (Optional)', below)
• ingest anything you collect
• use any non-disposable collecting equipment for food preparation or storage

 

Specimen Collection and Preparation Tips

We've chosen chloroplasts as the organelle of interest for this lab because they are relatively large and easily recognizable. If you can find your own specimens that clearly show chloroplasts, then that's great, but it's not strictly necessary. Flora or fauna, there's plenty to see with the foldscope, and the most important aspect about this part of the lab is that you get to experience the thrill of discovery!

Additional Equipment

We provide you with a foldscope, transfer pipette, and a pair of gloves. You should also gather together the following items:

• small container with a lid - pop bottle, etc., for wet specimens (spice jars from the dollar store work well)
• small plastic bag or envelope for dry specimens such as leaves, pollen, spores, etc. (but prepare them for viewing as soon as possible after collecting)
• toothpicks, plastic utensils, or other similar items (something you can scrape with) for taking small soil samples or collecting samples from the surface of rocks or trees
• a longer, thin stick such as a pencil, chopstick, barbeque skewer, etc. to safely extend your reach if necessary
• paper or a notebook and pencil
• camera
• tweezers (if you have them)
• a grocery bag or other container for collecting garbage

Algae and Plants

Your best chance for getting a good view of chloroplasts is to find some filamentous green algae. At your collecting site (see Collecting Tips: Locations, below), look for rocks or muddy patches with a greenish cast or film on the surface. In lakes, you may also see obvious clumps of filamentous algae that are either clinging to rocks or floating freely in the water (but see Notes of Caution, above). On shore, look for a green film on the surface of rocks. If you or someone you know has a fish tank, you might find some algae there.

Plant leaves may also work, but they must be very thin - if a specimen is more than a few cell layers thick, then it will be too thick for the cells to be visible. Choose leaves from plants that that are naturally very small (rather than using fragments of larger leaves), and look near the exterior margins of the leaf or near the veins, where the leaf tissue is typically thinner. Mosses and other ground-hugging weedy plants are good candidates, as are fish tank ornamentals such as Elodea.

If you collect a small amount of mud from a damp or marshy area, you have a good chance of finding motile organisms, even if you don't find algae. You may even want to try collecting a spoonful of soil in a glass jar, adding some water, and leaving it to sit in a sunny location. After a few days, you may have some algae growing in the jar! (If you try this, choose soil from a damp or shaded area with lots of plants growing).

Other Specimens to Try (Optional)

None of these have chloroplasts, but they could be fun to try...

• mushroom gills: whole mushrooms from a grocery store or the dining hall only - do no collect wild mushrooms!
• flower parts (petals, stamens, anthers): as with leaves, the smaller and thinner the better. There are lots of teeny tiny flowers out there if you look carefully!
• pollen grains: tap a flower head onto your foldscope slide
• fern sporangia (in season): look for the bumps on the underside of larger fern leaves; each bump is a sorus (pl. sori) that consists of many sporangia - gently scrape a sorus into your collecting envelope / bag
• onion skin (the moist, membranous inner layers, not the papery outer layer) - the cells of red onions are pigmented, and therefore easier to see
• lichen - macerate a small amount with a spoon or other blunt object in a small amount of water to increase your chances of seeing something recognizable  

 

Collecting Tips: Locations

On / Near Campus

• Ocean Pond - located between the Chase Building and the corridor between the LSC and Chemistry building. This is a small, marshy area that has been allowed to grow in naturally since it was established several years ago. Neither ocean nor pond, but you should at least find a puddle or two! If you don't find algae here, try collecting a small amount of mud and water.
• walkways - keep your eyes on the ground and look for algal growth on the compressed soil around the concrete / conglomerate pavers.
• South Street - follow South St. past Beaufort Avenue until it comes to an end at the Northwest Arm. There is a small rocky beach here where you may find some drift seaweed. If you see anything green and stringy, take a little bit! Do not enter the water.  
• Dalhousie Outdoor Ecolab (outer edges only) - in the wooded area behind Sheriff Hall, home of the Bill Freedman Plant Collection. While Dr. Freedman would be thrilled to see students studying this area closely, as of Fall 2018 it is in the early stages of being established. Please enjoy this area from the perimeter only, and don't walk through it until there is interpretive signage.

 

Further Afield

• The Halifax Regional Municipality manages a number of parks with beach access to freshwater lakes. Please note that lifeguard supervision is provided only during the summer months (May-August). Do not enter the water or collect specimens at any beaches or lakes where a risk advisory is in effect.
• Point Pleasant Park - Check the beach for green filamentous material among the seaweed that has washed ashore, or look for puddles and ponds in the interior of the park.
• Belcher's Marsh Park and Little Belcher's Pond Park - both accessible from Parkland Drive in Clayton Park; Little Belcher's has easier access to the water.

 

At the Site

Take a few photos of the site in general and the specific spot where you collect your specimen, safely collect the specimen, and make notes about the time, location, and weather conditions (see the assignment document attached to the dropbox for this lab).

If collecting wet or muddy specimens, nearly fill your specimen bottle with water from the site; then add your specimen. Or, bring some tap water with you, for algae that you may collect from relatively dry spots as well, such as the surface of rocks or soil. For sites on Halifax Harbour or the Northwest Arm, collect a small amount of water at the site. (This will be seawater; any specimens you collect that came from seawater should be stored in seawater, not fresh or tap water).

 

 

 

 

 

 

 

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Image Credits
All photos on this page by Jennifer Van Dommelen.

 

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